Background and Objectives: Plant nutrition is an important factor in the growth and chemical composition of plants. The application of natural fertilizers can enhance the performance and pharmacological properties of plants. Rosemary (Rosmarinus officinalis L. ) belonged to Lamiaceae family. Application of chemical fertilizers causes environmental pollution and ecological damage. One of the solutions to these problems is the use of sustainable agriculture principles in agroecosystems, including the use of organic fertilizers such as vermicompost and manure fertilizer. Considering the mentioned issues and taking into account the importance of rosemary herb and the management of weeds in agricultural landscapes, which is one of the biggest problems faced by farmers, this experiment was carried out with the aim of investigating the effect of fertilizer sources on biochemical characteristics and yield of rosemary in Competition and non-Competition with weeds conditions. Materials and Methods: This experiment was conducted as a split plot in a randomized complete blocks design in three replications at Sari Agricultural Sciences and Natural Resources University in 2016. The treatments consisted of Competition between plants and weeds (weedy and weed free) as a main factor and sub factor was fertilizer sources include sheep manure (20 ton ha-1), vermicompost (10 ton ha-1), sheep manure (10 ton ha-1) + vermicompost (5 ton ha-1), NPK fertilizer, NPK nanofertilizer and control (no fertilizer). The studied traits included: photosynthetic pigments (chlorophyll a, b, total and carotenoid), total phenol, total flavonoids, antioxidant activity, nutrient content (NPK), yield of rosemary and weeds biomass. Results: The results showed that treatments had a significant effect on all traits. highest levels of chlorophyll a (7. 24 μ g ml-1), chlorophyll b (2. 66 μ g ml-1), total chlorophyll (10. 08 μ g ml-1) and carotenoid (2. 19 μ g ml-1) was observed in vermicompost treatment, which was happen in weed free condition too. Maximum amount of total phenol (70. 85 mg g-1 leaf dry weight), total flavonoid (46. 43 μ g ml-1 leaf dry weight) and antioxidant activity (91%) were observed in sheep manure with weed interference, sheep manure with weed interference and vermicompost without weeding, respectively. While, maximum amounts of nitrogen (1. 65%) and potassium (1. 90%) were seen in vermicompost in weed free condition and for phosphorus (1. 26%) in sheep manure with weed free, respectively. The highest weeds biomass was observed in chemical fertilizer treatment in all three sampling. Conclusion: The results of the experiment indicated the superiority of organic fertilizers to chemical fertilizers, as well as the positive response of rosemary to fertilizer application. Non-use of chemical inputs in the production of medicinal plants and their products is a prerequisite for their health and naturalness. Therefore, considering the positive response of rosemary herb to the use of organic fertilizers, seems that the application of these fertilizers, reduced the use of chemical fertilizers and have no environmental consequences, decrease weeds biomass and reduces weed damage, as well as reducing herbicide use; therefore it is a good way to produce healthy and sustainable products of these species.